Technique for extracting niacin from Mycobacterium tuberculosis cultured on 7H-10 and 7H-11 agars.
نویسنده
چکیده
Niacin extractions from M. tuberculosis growing on 7H-10 or 7H-11 agar base medium yield consistent results when performed at 37 degrees C for 2 h. The technique does not require any modification of the media formulation.
منابع مشابه
Activity and penetration of antituberculosis drugs in mouse peritoneal macrophages infected with Mycobacterium microti OV254.
The activities of some commonly used antituberculosis drugs were investigated within unstimulated peritoneal macrophages and in 7H-9 medium without Tween 80 using Mycobacterium microti OV254 as the target organism. In macrophage cultures, serial concentrations of isoniazid, rifampin, pyrazinamide, or streptomycin were added after a 2.5-h phagocytosis period. Viable counts were carried out at da...
متن کاملIsolation of acid-fast organisms from surgical specimens.
A total of 204 surgical specimens positive for acid-fast organisms yielded 169 strains of Mycobacterium tuberculosis, 15 of which were isolated by Middlebrook 7H-9 broth medium only. Mycobacteria other than M. tuberculosis were isolated from 33 specimens, and Nocardia asteroides was isolated from 2 specimens.
متن کاملتشخیص سریع مایکوباکتریوم‌های آتیپیک در بیماران با علایم سل ریوی: ارزیابی لوکوس (QUB 3232 (590bp با روش VNTR
Background and Objective: Identification of atypical mycobacterium (Non tuberculosis Mycobacterium NTM) is important because of the worldwide propagation of these organisms. Recently, molecular studies have identified the specific loci for mycobacterium species by DNA - finger printing methods, but these methods are time-consuming and expensive. In this study, in addition to hsp65 PCR-RFLP meth...
متن کاملارزش تشخیصی تست gyrB-RFLP PCR در تعیین گونه مایکوباکتریوم‌های بیماریزا در بیماران مسلول در استان مازندران
Background and purpose: Mycobacterium tuberculosis complex (MTBC) members are causative agents of human and animal tuberculosis. Differentiation of MTBC members is essential for appropriate treatment of individual patients and reduce drug resistance. Materials and methods: A total of 1345 samples were collected from patients clinically suspected of contracting tuberculosis that referred to hea...
متن کاملIn vitro effect of rifampin on mycobacteria.
Rifampin inhibited 20 strains of Mycobacterium tuberculosis in concentrations of 0.005 to 0.02 mug/ml in 7H-9 broth with Tween 80 and killed all or nearly all of the inoculum in four to eight times greater concentrations. In the same medium without Tween 80, as well as on 7H-10 agar, about 16 to 64 times these amounts were required to produce the same effect. Rifampin was also active against M....
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
عنوان ژورنال:
- Journal of clinical microbiology
دوره 15 5 شماره
صفحات -
تاریخ انتشار 1982